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Cell lysis is the critical step
for plasmid production |
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VGXI considers lysis a purification
step with 99.9% of the mass removed |
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We Avoid any human intervention
in the process |
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We Avoid centrifugation or any
high shear on the resulting lysate to minimize genomic contamination |
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We do not add animal-derived enzyme |
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It is well known that batch processing
of lysate by hand yields variable results |
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We test several lysis production
rates early to determine scalability |
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At VGXI, we utilize a proprietary
Continuous Capture Method. |
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This method allows us to effectively
lyses cells at a continuous high rate without damaging cellular
contents |
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Neutralize, mix, separate, and
remove solids in one simple operation, without the need for
centrifugation or bag filtration |
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Simultaneous primary capture plasmid
product to create a semi pure process stream |
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